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MCQ on Biotechnology - Plant Tissue Culture



1. Who is the father of tissue culture?
a) Bonner
b) Haberlandt
c) Laibach
d) Gautheret
2. The production of secondary metabolites require the use of
a) protoplast
b) cell suspension
c) meristem
d) auxillary buds
3. Synthetic seed is produced by encapsulating somatic embryo with
a) sodium chloride
b) sodium alginate
c) sodium acetate
d) sodium nitrate
4. Hormone pair required for a callus to differentiate are
a) auxin and cytokinin
b) auxin and ethylene
c) auxin and absiccic acid
d) cytokinins and gibberllin
5. DMSO (Dimethyl sulfoxide) is used as
a) Gelling agent
b) alkaylating agent
c) Chelating agent
d) Cryoprotectant
6. The most widely used chemical for protoplast fusion, as fusogens, is
a) Manitol
b) Sorbitol
c) Mannol
d) Poly ethylene glycol (PEG)
7. Cybrids are produced by
a) Fusion of two different nuclei from two different species
b) Fusion of two same nuclei from same species
c) Nucleus of one species but cytoplasm from both the parent species
d) None of the above
8. Callus is
a) Tissue that forms embryo
b) An insoluble carbohydrate
c) Tissue that grows to form embryoid
d) Un organised actively dividing mass of cells maintained in cultured
9. Part of plant used for culturing is called
a) Scion
b) Explant
c) Stock
d) Callus
10. Growth hormone producing apical dominance is
a) Auxin
b) Gibberellin
c) Ethylene
d) Cytokinin
11. A medium which is composed of chemically defined compound is called
a) Natural media
b) Synthetic media
c) Artificial media
d) None of these
12. To obtain haploid plant, we culture
a) Entire anther
b) Nucleus
c) Embryo
d) Apical bud
13. Somaclonal variations are the ones
a) Caused by mutagens
b) Produce during tissue culture
c) Caused by gamma rays
d) Induced during sexual embryogeny
14. Which of the following plant cell will show totipotency?
a) Xylem vessels
b) Sieve tube
c) Meristem
d) Cork cells
15. Which vector is mostly used in crop improvement?
a) Plasmid
b) Cosmid
c) Phasmid
d) Agrobacterium
Answers:

  1. b) Haberlandt
  2. b) cell suspension
  3. b) sodium alginate
  4. a) auxin and cytokinin
  5. d) Cryoprotectant
  6. d) Poly ethylene glycol (PEG)
  7. c) Nucleus of one species but cytoplasm from both the parent species
  8. d) Un organised actively dividing mass of cells maintained in cultured
  9. b) Explant
  10. a) Auxin
  11. b) Synthetic media
  12. a) Entire anther
  13. b) Produce during tissue culture
  14. c) Meristem
  15. d) Agrobacterium