1. A recombinant DNA molecule is produced by
6. Who discovered restriction enzymes
2. The gene formed by the joining of DNA segments from two different sources are called asa) joining of two DNA fragments
b) joining of two or more DNA fragments
c) both a and b
d) joining of two or more DNA fragments originating from different organisms
a) recombinant gene3. Which of the following enzyme is used to cut DNA molecule in rDNA technology
b) joined gene
c) both a and b
d) chimaeric gene
a) ligase4. Restriction enzymes are also called as
b) phosphatase
c) ribonuclease
d) restriction enzymes
a) biological scissors5. The most important discovery that lead to the development of rDNA technology was
b) molecular scalpels
c) molecular knives
d) all of these
a) Double helix model of Watson and Crick
b) discovery of restriction enzymes
c) discovery of ligase enzyme
d) discovery of plasmids
6. Who discovered restriction enzymes
a) Nathan, Arber and Smith in 19707. Who created the first rDNA molecule
b) Watson, Crick and Wilkins in 1970
c) Boyer and Cohen in 1975
d) Paul Berg in 1975
a) Nathan, Arber and Smith
b) Watson, Crick and Wilkins8. The DNA molecule to which the gene of insert is integrated for cloning is called
c) Boyer and Cohen
d) Paul Berg
a) carrier9. The DNA segment to be cloned is called
b) transformer
c) vector
d) none of these
a) gene segment10. Which of the following statements are true regarding rDNA technology
b) DNA fragment
c) DNA insert
d) all of these
a) rDNA technology is used to obtain large number of copies of specific DNA fragments11. The first successful transformation of rDNA molecule into a bacterium was carried out by
b) rDNA technology is used to obtain large quantities of the protein produced by the concerned gene
c) rDNA technology is used to integrate gene of interest into chromosomes where it expresses itself
d) all of these
a) Nathan, Arber and Smith12. The plasmid used by Cohen and Boyer for their transformation experiment was
b) Watson, Crick and Wilkins
c) Boyer and Cohen
d) Paul Berg
a) pSC 10113. The mechanism of intake of DNA fragments from the surrounding medium by a cell is called
b) PUC 17
c) pBR 322
d) E.coli plasmids
a) transformation14. Gene cloning refers to the
b) transduction
c) both a and b
d) conjugation
a) production of large number of copies of the gene being cloned15. Paul Berg’s gene splicing experiment created the first rDNA molecule which was a
b) production of asexual progeny from a single individual or a cell
c) both a and b
d) none of these
a) a T4 phage fragment incorporated into SV40 vector
b) a lambda phage fragment incorporated into SV40 vector
c) a T4 phage fragment incorporated into pSC 101 vector
d) a lambda phage fragment incorporated into pSC 101 vector
Answers:
1. d) joining of two or more DNA fragments originating from different organisms
2. d) chimaeric gene
3. d) restriction enzymes
4. a) biological scissors
5. b) discovery of restriction enzymes
6. a) Nathan, Arber and Smith in 1970
7. d) Paul Berg
8. c) vector
9. c) DNA insert
10. d) all of these
11. c) Boyer and Cohen
12. a) pSC 101
13. a) transformation
14. a) production of large number of copies of the gene being cloned
15. b) a lambda phage fragment incorporated into SV40 vector