MCQ on Recombinant DNA Technology

1. A recombinant DNA molecule is produced by 

a) joining of two DNA fragments 
b) joining of two or more DNA fragments 
c) both a and b 
d) joining of two or more DNA fragments originating from different organisms

2. The gene formed by the joining of DNA segments from two different sources are called as

a) recombinant gene 
b) joined gene 
c) both a and b 
d) chimaeric gene

3. Which of the following enzyme is used to cut DNA molecule in rDNA technology 

a) ligase 
b) phosphatase 
c) ribonuclease 
d) restriction enzymes

4. Restriction enzymes are also called as 

a) biological scissors 
b) molecular scalpels 
c) molecular knives 
d) all of these

5. The most important discovery that lead to the development of rDNA technology was 

a) Double helix model of Watson and Crick 
b) discovery of restriction enzymes 
c) discovery of ligase enzyme 
d) discovery of plasmids

6. Who discovered restriction enzymes 

a) Nathan, Arber and Smith in 1970 
b) Watson, Crick and Wilkins in 1970 
c) Boyer and Cohen in 1975 
d) Paul Berg in 1975

7. Who created the first rDNA molecule 

a) Nathan, Arber and Smith

b) Watson, Crick and Wilkins 
c) Boyer and Cohen 
d) Paul Berg

8. The DNA molecule to which the gene of insert is integrated for cloning is called 

a) carrier 
b) transformer 
c) vector 
d) none of these

9. The DNA segment to be cloned is called 

a) gene segment 
b) DNA fragment 
c) DNA insert 
d) all of these

10. Which of the following statements are true regarding rDNA technology 

a) rDNA technology is used to obtain large number of copies of specific DNA fragments 
b) rDNA technology is used to obtain large quantities of the protein produced by the concerned gene 
c) rDNA technology is used to integrate gene of interest into chromosomes where it expresses itself 
d) all of these

11. The first successful transformation of rDNA molecule into a bacterium was carried out by 

a) Nathan, Arber and Smith 
b) Watson, Crick and Wilkins 
c) Boyer and Cohen 
d) Paul Berg

12. The plasmid used by Cohen and Boyer for their transformation experiment was 

a) pSC 101 
b) PUC 17 
c) pBR 322 
d) E.coli plasmids

13. The mechanism of intake of DNA fragments from the surrounding medium by a cell is called 

a) transformation 
b) transduction 
c) both a and b 
d) conjugation

14. Gene cloning refers to the 

a) production of large number of copies of the gene being cloned 
b) production of asexual progeny from a single individual or a cell 
c) both a and b 
d) none of these

15. Paul Berg’s gene splicing experiment created the first rDNA molecule which was a

a) a T4 phage fragment incorporated into SV40 vector 
b) a lambda phage fragment incorporated into SV40 vector 
c) a T4 phage fragment incorporated into pSC 101 vector 
d) a lambda phage fragment incorporated into pSC 101 vector

Answers:
1. d) joining of two or more DNA fragments originating from different organisms
2. d) chimaeric gene
3. d) restriction enzymes
4. a) biological scissors
5. b) discovery of restriction enzymes
6. a) Nathan, Arber and Smith in 1970
7. d) Paul Berg
8. c) vector
9. c) DNA insert
10. d) all of these
11. c) Boyer and Cohen
12. a) pSC 101
13. a) transformation
14. a) production of large number of copies of the gene being cloned
15. b) a lambda phage fragment incorporated into SV40 vector