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Multiple Choice Questions on Biotechnology

1. Genetic engineering is possible, because 
a) We can cut DNA at specific sites by endonucleases like DNAase I 
b) Restriction endonucleases purified from bacteria can be used in vitro 
c) The phenomenon of transduction in bacteria is well understood 
d) We can see DNA by electron microscope
2. Restriction endonucleases (Restriction enzymes) are used for
Genetic Engineering
a) Used to invitro DNA synthesis
b) Used in genetic engineering
c) Synthesized by bacteria
d) Present in mammalian cells for degradation of DNA

3. Transgenic animals are those which are 
a) Foreign RNA in all its cells 
b) Foreign DNA in some of its cells 
c) Foreign DNA in all its cells 
d) Both a and b

4. The first transgenic plant was
a) Tobacco
b) Pea
c) Flax
d) Cotton

5. Which of the following is related to genetic Engineering? 
a) Plastid
b) Plasmid
c) Heterosis
d) Mutation

6. First cloned animal 
a) Dolly sheep
b) Dog
c) Mule
d) Cat

7. Which of the following bases in plasmids discovered so far 
a) 50 kilo base
b) 500 kilo base
c) 5 kilo base
d) 500 kilo base


8. Plasmid has been used as vector because 
plasmid
a) It has antibiotic resistance gene 
b) It is circular DNA which have capacity to join to eukaryotic DNA 
c) Both ends show replication 
d) It can move between prokaryotic and eukaryotic cells

9. The bacteria generally used for genetic engineering is 
a) Bacillus 
b) Pseudomonas 
c) Clostridium 
d) Agrobacterium

10. Which of the following cut the DNA from specific places 
a) E.coli restriction endonuclease II
b) Ligase 
c) Exonuclease 
d) Alkaline phosphatase
    Answers 
    1. b) Restriction endonucleases purified from bacteria can be used in vitro 
    2. b) Used in genetic engineering 
    3. c) Foreign DNA in all its cells 
    4. a) Tobacco 
    5. b) Plasmid 
    6. a) Dolly sheep 
    7. b) 500 kilo base 
    8. b) It is circular DNA which have capacity to join to eukaryotic DNA 
    9. d) Agrobacterium
    10.a) E.coli restriction endonuclease II


    1. The work on GFP, widely used as a reporter gene, won the Nobel Prize in: 
    a) Medicine/Physiology-2000 
    b) Chemistry-2008 
    c) Physics-2008 
    d) Biochemistry-2007

    2. The method of plasmid isolation by alkaline lysis was published by 

    a) Mandel and Higa 
    b) Sharp and Lederberg 
    c) Temin and Baltimore 
    d) Birnboim and Doly

    3. Alec Jeffery’s name is associated with 

    a) DNA sequencing 
    b) DNA Fingerprinting 
    c) RNA sequencing 
    d) Site directed mutagenesis

    4. The name Kary Mullis is associated with 

    a) RFLP 
    b) PCR 
    c) Chain Termination reaction 
    d) RAPD

    5. The group associated with first man made recombinant DNA molecules: 

    a) Daniel Nathans, Arber, Kary Mullis 
    b) Paul Berg, Annie Chang, Boyer, Stanley Cohen 
    c) Howard Temin, Sydney Brenner, Philip Sharp 
    d) Tim Hunt, Paul Nurse, Leyland Hartwell

    6. The 2006, Nobel Prize for RNA interference phenomenon was won for the work carried out on: 

    a) D melanogaster 
    b) A thalliana 
    c) C. elegans 
    d) S pombe

    7. GFP protein was originally isolated from 

     GFP -Aequoria victoria 
    a) Arabidopsis thaliana 
    b) Aequoria victoria 
    c) C elegans 
    d) Drosophila melanogaster


    8. The first crop plant genome sequenced 
    a) Maize 
    b) Wheat 
    c) Rice 
    d) Barley

    9. The first transgenic plant to be produced 

    a) Rice 
    b)Maize 
    c) Cotton 
    d) Tobacco

    10.The year of launching of Human Genome Project and completion of rough draft of the sequence was in 

    a) 1992-2000 
    b) 1990-2000 
    c) 1990-2001 
    d) 1991-2001

    Answers
    1. b) Chemistry-2008
    2. d) Birnboim and Doly
    3. b) DNA Fingerprinting
    4. b) PCR
    5. b) Paul Berg, Annie Chang, Boyer, Stanley Cohen
    6. c) C. elegans
    7. b) Aequoria victoria
    8. c) Rice
    9. d) Tobacco
    10. b) 1990-2000



    11) A protein is poorly expressed in a diseased tissue. To determine whether the defect is at the level of transcription or translation, which of the following blotting techniques would you use?
    a) Southern and Western
    b) Southern and Northern
    c) Northern and Western
    d) Western and South –Western

    12) The polymerase chain reaction (PCR)
    a) is used to transcribe specific genes
    b) amplifies specific DNA sequences
    c) uses a DNA polymerase that denatures at 55 0 C
    d) is a method for sequencing DNA

    Steps involved in PCR 


    13) Genome wise gene expression analysis is performed using
    a) DNA microarrays
    b) Northern analysis
    c) Real time PCR
    d) RT-PCR

    14) Triton X-100 is a surfactant that forms micellar structure in aqueous solutions. One can form reverse micelles of the surfactants easily by
    a) Addition of salts
    b) Making the pH acidic from alkaline
    c) Addition of heavy metal ions
    d) Addition of non polar solvents

    15) SDS is used in polyacrylamide gel electrophoresis of a mixture of proteins for their efficient separation on the gel. SDS, in this experiment, is used to
    a) Solubilise the protein
    b) Stabilize the protein
    c) decrease the surface tension of buffer
    d) have uniform charge density on the proteins

    16) Exchange of Germplasm is carried out preferably through shoot tip culture because they are
    a) Virus free
    b) Germplasm is present at shoot tip only
    c) Shoot tip is disease free
    d) All of these

    17) Which of the following is the best method to determine bacteriophages concentration in a sample?
    a) Spectrophotometry
    b) Plaque assay
    c) Copy assay number
    c) Light microscopy

    18) Insertional inactivation of a gene helps in
    a) Identification of recombinant clones
    b) Identification of deletion mutants
    c) Identification of suppression mutants
    d)elimination of recombinant clones

    19) Electroporation facilitates introduction of foreign DNA into the target organism by
    a) Changing the porosity of the cell wall
    b) Changing the electric potential of the cell wall
    c) Lysis of the cell wall
    d) Active transport across the cell wall

    20) A method for transferring protein to a nitrocellulose filter on which protein can be detected by a suitable probe is:
    a) Southern blotting
    b) Northern Blotting
    c) Western blotting
    d) None of these

    Answers
    11. d) Western and South -Western
    12. b) amplifies specific DNA sequences
    13. a) DNA microarrays
    14. d) Addition of non polar solvents
    15. d) have uniform charge density on the proteins
    16. a) Virus free
    17. b) Plaque assay
    18. a) Identification of recombinant clones
    19. a) Changing the porosity of the cell wall
    20. c) Western blotting